Characterization of extracellular protease from halophilic microbes isolated from Bledug Kuwu, Grobogan, Central Java

High salt-tolerant extracellular protease is an enzyme that plays a crucial role in industries and requires high salt conditions. Some enzymes are not resistant to conditions because they may denature under concentrations. Halophilic microbes produce stable at salts. This research has succeeded isolating several halophilic capable of producing proteases from Bledug Kuwu, Grobogan, Central Java, Indonesia. Extracellular bacteria screened with skim milk selective medium purified the quadrant streak method. Then, pure isolate resulting were characterized BK1B was Gram-positive bacterium, while BK1D Gram-negative bacterium. halotolerant bacterium moderate had largest proteolytic index values, optimal production time three days pH 6. worked optimally 6 temperatures 45 42 °C 2.541 3.505 U/mL activity resistance NaCl concentrations ranging 3 - 13% NaCl. suggests strains can be